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p62 sequestered Keap1 into aggregates. (A) The cellular localization of p62, Keap1, Nrf2, and Cul3. HEK293 cells were singly transfected with an expression vector for the fluorescently tagged protein. The subcellular localization of the proteins was monitored live. (B and C) The colocalization of proteins. Live imaging of the proteins was monitored with HEK293 cells that were cotransfected with expression vectors for the indicated fluorescently tagged proteins.
