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. 2010 Apr 26;30(13):3275–3285. doi: 10.1128/MCB.00248-10

FIG. 5.

FIG. 5.

Autophagy-defective cells sequestered Keap1 into aggregates. (A and B) Keap1 was sequestered into aggregates in primary autophagy-deficient cells. Atg5+/+, Atg5−/−, Beclin1+/+, and Beclin+/− iBMK cells stably expressing GFP alone or p62-GFP were transfected with CFP-tagged Keap1. Live imaging was taken 24 h posttransfection. (C and D) Endogenous Keap1 was sequestered into aggregates in primary autophagy-deficient cells. The cells were grown on coverslips and fixed in methanol. Indirect immunofluorescence staining was conducted using an antibody against GFP and Keap1.