TABLE 1.
Primers used for mutating Marek's disease virus genes in transmission-competent pRB-1B
| Mutanta | Directionb | Sequencec |
|---|---|---|
| ΔUS2 | Forward | CAGTTATTAACAATAAAAAAGATTATTGGTGGAGGTGAAGTAGAATTCAGATCTGCTAGATAGGGATAACAGGGTAATCGATTT |
| Reverse | GCATACATTATACGAAGTTATCTAGCAGATCTGAATTCTACTTCACCTCCACCAATAATCGCCAGTGTTACAACCAATTAACC | |
| US2M1 stop | Forward | CCCAGTTATTAACAATAAAAAAGATTATTGGTGGAGGTGAAGTAAGGTGTGTCCATGATAACTATTAGGGATAACAGGGTAATCGATTT |
| Reverse | ATCGCATTCATCTAGAAGTGTGACTATAGTTATCATGGACACACCTTACTTCACCTCCACCAATAGCCAGTGTTACAACCAATTAACC | |
| UL13 K170M | Forward | CGGAGTAGTTAAAATATTTAAGAAGACGGACATAGCCGTCATGAAGTATTGGAATGTTTTAATAGGGATAACAGGGTAATCGAT |
| Reverse | ATGTCATAAGTAACTCAGTTTTAAAACATTCCAATACTTTCATGACGGCTATGTCCGTCTTCTGCCAGTGTTACAACCAATTAAC | |
| UL13 M170K | Forward | CGGAGTAGTTAAAATATTTAAGAAGACGGACATAGCCGTCAAAAAAGTATTGGAATGTTTTAATAGGGATAACAGGGTAATCGAT |
| Reverse | ATGTCATAAGTAACTCAGTTTTAAAACATTCCAATACTTTTTTGACGGCTATGTCCGTCTTCTGCCAGTGTTACAACCAATTAAC | |
| UL13 K270A | Forward | TTCTAAACGTGTCTTGTGGGTTGACTCATTTGGATATCGCATGTGGGAATATCTTTGGCCAGTGTTACAACCAATTAACC |
| Reverse | AGGACCCTCGGTGACGTTAACAAAGATATTCCCACATGCGATATCCAAATGAGTCATAGGGATAACAGGGTAATCGATTT | |
| gCM1 stop | Forward | CCAAACGTAACCCTCTACATATCTTCCCTCTAGCTCACGCCGCGTGTTTTACGAGCTTTGTAGGGATAACAGGGTAATCGATTT |
| Reverse | AAAAAGAGTCCAGTCCACCCCAAAGCTCGTAAAACACGCGGCGTGAGCTAGAGGGAAGATGCCAGTGTTACAACCAATTAACC | |
| gCstopM1 | Forward | CCAAACGTAACCCTCTACATATCTTCCCTCATGCTCACGCCGCGTGTGTTACGAGCTTTGTAGGGATAACAGGGTAATCGATTT |
| Reverse | AAAAAGAGTCCAGTCCACCCCAAAGCTCGTAACACACGCGGCGTGAGCATGAGGGAAGATGCCAGTGTTACAACCAATTAACC |
a
Gene mutation.
b
Directionality of the primer.
c
Underlined sequence indicates the template binding region of the primers for PCR amplification with pEPKanS. Bold and italicized letters indicate the mutated sequences introduced into the genome.