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TEM ultrastructural confirmation of autophagic vacuoles. 293T cells were transfected with pCMVtag (A) as a control or with pCMVtagORF50 (RTA) (B) and were cultured under nutrient-rich conditions. Arrows indicate autophagic vacuoles. N, nucleus. Panels B1, B2, and B3 are magnified views of autophagic vacuole structures from the boxed regions shown in panel B. M, mitochondria. Scale bars = 1 μm.
