FIG. 3.
Alignment of the gp120 amino acid sequences from the indicated passages in the NBD-556 and sCD4 escape processes. The amino acid sequences were deduced from the nucleotide sequences of the env-encoding regions of proviral DNA isolated from cells infected with the HIV-1IIIB variants selected in the presence of NBD-556 (A) or sCD4 (B) and the passage control. The amino acid sequences of the envelope proteins of the baseline HIV-1IIIB are shown at the top as a reference. The identity of the sequences at the individual amino acid positions is indicated by dots. The numbers of clones with the given amino acid substitutions among a total of 8 to 12 clones are listed. The number in parentheses denotes the concentrations of NBD-556 or sCD4. The major mutations of NBD-556 and sCD4-resistant variants at final passage are boxed.