FIG. 5.

Sensitivities of β-galactosidase reporter HIV strains pseudotyped with the sCD4 and NBD-556 resistance envelope mutations to NBD-556, YYA-004, sCD4, and MAbs. The sensitivities of β-galactosidase reporter HIV strains pseudotyped with the sCD4 and NBD-556 resistance envelope mutations to NBD-556 (A), YYA-004 (B), sCD4 (C), 2G12 (D), b12 (E), and RPA-T4 (F) are shown. NBD-556, YYA-004, sCD4, and MAbs at various concentrations and a pseudovirus suspension corresponding to 100 TCID₅₀ were preincubated for 15 min on ice and then added to the target cells (TZM-bl). The inhibitory effects were determined by measuring the β-galactosidase activities on day 2 of culture. All assays were conducted in triplicate, and the data shown represent the means ± the standard deviations (SD) derived from the results of two to three independent experiments.