FIG. 2.

Binding of MK2 to the RNase H active site of RT. Close-up view of MK2 (cyan) bound to the active site. The two Mn²⁺ ions are shown in green, and bonds to the Mn²⁺ ions are shown in blue. One Mn²⁺ is coordinated by carbonyl oxygens from D443 and D549 and two oxygens of the naphthyridinone. The other Mn²⁺ is coordinated by the other carbonyl oxygen of D443, E478, D498, and the naphthyridine moiety of the compound. In addition to contacts with the metal ions, the inhibitor also interacts with a loop in RT containing A538 and H539. Select protein 1:1 inhibitor bonds are shown in black.