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ThermoFluor analysis of metal dependence on inhibitor binding to full-length RT. Fluorescence-based thermal denaturation temperatures for RT in the absence (DMSO control) or presence of inhibitor. Experiments are conducted in the absence of metal (blue), the presence of Mg2+ (yellow), or the presence of Mn2+ (purple). There is a metal-dependent increase in thermal stability for all three inhibitors.
