FIG. 6.
The yield of influenza virus is reduced in hStau1-silenced HEK293T cells. (A) The levels of hStau1 in WT HEK293T cells, TM clone cells, and the hStau1-silenced cell clones 1-4 and 1-8 were determined by Western blotting using actin as a standard. The two hStau1 isoforms are indicated by arrows, while the asterisk denotes a cross-reaction of the anti-hStau1 antibody. (B) Cultures of HEK293T cells (WT), cells silenced with the irrelevant TM sequence (TM), and the hStau1-silenced clones 1-4 and 1-8 were infected with WSN virus at an MOI of 10−3 PFU/cell, and aliquots of the supernatants were sampled at the indicated times after infection. Virus titers and standard deviations are presented, as determined by plaque assay with MDCK cells.