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. 2010 May 26;84(15):7603–7612. doi: 10.1128/JVI.00504-10

FIG. 7.

FIG. 7.

The yield of influenza virus is specifically reduced in A549 cells. (A) The level of hStau1 in A549 cells transfected with an irrelevant siRNA (control) or with 3 different siRNAs specific for hStau1 (S1, S2, and S3) was determined by Western blotting using actin as standard. (B) Cultures of A549 cells transfected with the siRNAs were infected with WSN virus at an MOI of 10−3 PFU/cell, and aliquots of the supernatants were sampled at the indicated times after infection. Virus titers and standard deviations are presented, as determined by plaque assay in MDCK cells. (C) The level of hStau1 was determined as described in the legend for panel A. (D) Cultures of A549 cells transfected with the siRNAs were infected with VSV at an MOI of 10−3 PFU/cell, and aliquots of the supernatants were sampled at the indicated times after infection. Virus titers and standard deviations are presented, as determined by plaque assay in BHK21 cells.