FIG. 9.

Production of virus particles in hStau1-silenced cells. Cultures of A549 cells were transfected with 3 different siRNAs specific for hStau1 (S1, S2, and S3) or an irrelevant siRNA as a control (C) and used for low-multiplicity infections with WSN influenza virus. Virus particles were purified from the supernatant media, and total cell extracts were prepared in Laemmli buffer. (A) Equal aliquots of the purified virions were analyzed by PAGE and Western blotting with anti-NP or anti-M1 antibodies. (B) Samples from the cell extracts were analyzed by PAGE and Western blotting with anti-NP (NP) or anti-actin antibodies (Actin). C/2 denotes loading one half the amount than in the control (C). (C) HEK293T cells (WT) and iStau clone 1-4 (C 1-4) cells were infected at a high MOI, and after 7 h, the supernatant was used to purify viral particles. Virion RNAs were purified and analyzed by denaturing polyacrylamide-urea gel electrophoresis and silver staining. The positions of the various RNA segments are indicated on the left.