FIG. 4.

Cytoplasm trafficking toward the nucleus. (A) Inhibitors of the endosomal pathway and acidification (light shaded bars), microtubules (filled bars), and actin networks (dark shaded bars) were used to evaluate their necessity for PPV infection. Shown are the relative percentages of infection at 20 h p.i. in the presence of optimal concentrations of the inhibitors as measured by indirect IF, as described for Fig. 2. (B) Impacts of the inhibitors on genome replication as measured by qPCR and normalized to cell numbers, as described for Fig. 3 (**, P < 0.003 by the t test). (C) Pulse inhibition. Inhibitors were added at different times p.i. and were left until 20 h p.i. The percentages of infected cells were determined (*, P < 0.03 by the t test for the difference between the Noc and LatA curves).