FIG. 7.

Analysis of the absolute numbers of splenocytes that express mLANA and the distribution of mLANA-expressing B cells over time. (A) The absolute numbers of cells that expressed mLANA versus cells that harbored viral genome. For each time point, the absolute number of splenocytes that expressed mLANA (black columns) was calculated from the percentage of mLANA-positive cells per spleen and the total number of splenocytes, as detailed in Table 1. Also shown are the absolute number of viral-genome-positive cells, as determined for Fig. 3, and the total number of splenocytes, as detailed in Table 1. (B) The distribution of CD19⁺ B cells that expressed mLANA. The percentage of CD19⁺ mLANA⁺ cells that displayed markers of naïve, germinal-center, or memory B cells was calculated based on the absolute number of mLANA⁺ cells in each population, as detailed in Table 1. Cells that were not accounted for in these calculations were termed “other.”