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. 2010 May 19;84(15):7683–7694. doi: 10.1128/JVI.02604-09

FIG. 8.

FIG. 8.

Effects of p17 on the phosphorylation of translation factors. DF-1 cells were transfected with p17 gene or pcDNA3.1 only. Cells were harvested for Western blot assay at the indicated time points. (A) The levels of phosphorylated Mnk1, eIF4E, eIF4B, eIF4G, eEF2K, and p70S6K notably decreased, while phosphorylation of eEF2 and eIF2α increased with time of p17 transfection. (B) Phosphorylation status of eIF4B and eIF4E in caffeine-treated (left upper panel) and caffeine-treated (right upper panel) p17-transfected cells at the indicated time points. The lower panels show the phosphorylation status of eIF4E in pcDNA3.1- and p53−/−-cotransfected DF-1 cells (left lower panel) and p17- and p53−/−-cotransfected DF-1 cells (right lower panel). eIF4E phosphorylation increased with transfection time despite caffeine treatment. The increase in phosphorylation level was exclusive to each treatment and was based on the zero time point as a control. The figure is representative from triplicate experiments. The numbers below the phosphorylated forms of the proteins indicate the densitometry data of band analysis. The data were expressed as percentages of the zero time point expression of the indicated proteins.