TABLE 1.
Primers used for gene amplification in this study
| Gene | Sequencea (5′-3′) | Location |
|---|---|---|
| P17 | CGTTGGATCCTGGTAAGCACAATGCAATGGCTCCG | 1-103 |
| CTTCAAGCTTGGTCAGCCGTTCATAGATCG | 104-146 | |
| σC | AGCGAATTCATGGCGGGTCTCAATCCATC | 630-649 |
| AGCAAGCTTTTAGGTGTCGATGCCGGTAC | 1591-1600 | |
| σA | CGCGGATCCACGATGGCGCGTGCCATATACG | 9-34 |
| AATAAGCTTACTTACGACCCTACGCCTAG | 1263-1279 | |
| σB | CGGAATTCGCAATGGAGGTA | 27-39 |
| GGGAATTCTGGGTGGGAGTC | 1171-1160 | |
| σNS | TTGAATTCTTGTGCAGCCATGGAC | 5-29 |
| GCGAATTCTCACCCGCACCATGGG | 1127-1143 | |
| p21cip1/waf1 (p21-BD2) | GGTGAATTCCTCTGAAAGCTGACTGC | 2613-2590 |
| CGGAGTGGAGTAAGTTCGTCTAGG | 535-560 | |
| p21cip1/waf1 (p21-BD1) | TGTGTCCAGCGCACCAGCAG | 273-252 |
| GTCTCCGCTTGGCTGGCAACC | 2414-2435 | |
| p21cip1/waf1 (p21-BD0) | TGTGTCCAGCGCACCAGCAG | 2613-535 |
| CGGAGTGGAGTAAGTTCGTCTAGG | 273-2414 |
a
The restriction site sequences for the respective primer sets are underlined.