Skip to main content
. 2010 May 26;84(15):7713–7725. doi: 10.1128/JVI.00310-10

FIG. 1.

FIG. 1.

Experiments to demonstrate miRNA activity on helper RNAs containing matched target complements (3′ UTR positive-strand RNA). (A) Schematic of the Δ26S miRNA-targeted helper RNAs. (B) Capsid- and GP-specific Western blot analysis of cell protein lysates collected 18 h postelectroporation with miRNA-targeted helper RNAs and a replicon RNA in the absence or presence of unmatched or matched complements of miRNA inhibitors. (C) Capsid- and GP-specific Northern blot analysis of total cellular RNA collected 18 h postelectroporation with miRNA-targeted helper RNAs and a replicon RNA in the absence or presence of unmatched or matched complements of miRNA inhibitors. (D) IFA titer of VRP generated with miRNA-targeted helper RNAs and a replicon RNA in the absence or presence of unmatched or matched complements of miRNA inhibitors. Cont, negative control Vero cell lysates.