FIG. 2.

Experiments to demonstrate miRNA activity on helper RNAs containing unmatched target complements (3′ UTR positive-strand RNA). (A) Capsid- and GP-specific Western blot analysis of cell protein lysates collected 18 h postelectroporation with miRNA-targeted helper RNAs and a replicon RNA in the absence or presence of matched complements of miRNA inhibitors. (B) Capsid- and GP-specific Northern blot analysis of total cellular RNA collected 18 h postelectroporation with miRNA targeted helper RNAs and a replicon RNA in the absence or presence of matched complements of miRNA inhibitors. (C) IFA titer of VRP generated with miRNA-targeted helper RNAs and a replicon RNA in the absence or presence of matched complements of miRNA inhibitors.