FIG. 4.

Experiments to determine the contribution of individual miRNA targets on capsid helper RNA replication in Vero cells (six individual miRNA copies in tandem on the 3′ UTR positive-strand RNA). (A) Capsid-specific Western blot analysis of cell protein lysates collected 18 h postelectroporation with miRNA-targeted capsid helper RNAs and a replicon RNA in the absence or presence of matched complements of miRNA inhibitors. (B) Capsid-specific Northern blot analysis of total cellular RNA collected 18 h postelectroporation with miRNA-targeted capsid helper RNAs and a replicon RNA in the absence or presence of matched complements of miRNA inhibitors. (C) IFA titer of VRP generated with miRNA-targeted capsid helper RNAs and a replicon RNA in the absence or presence of matched complements of miRNA inhibitors.