FIG. 2.
Coimunoprecipitation of proteins with pUL17-specific antibody. Rabbit skin cells were infected with HSV-1(F) or the UL17 deletion virus, and pUL17 was immunoprecipitated. Immunoprecipitated proteins were electrophoretically separated on a 15% (A) or 8% (B and C) SDS-polyacrylamide gel and stained with Coomassie brilliant blue or were subjected to immunoblotting with pUL17-specific antibody as indicated. Proteins unique to or highly enriched in immunoaffinity purifications from HSV-1(F)-infected cell lysates are indicated with arrows, and their identities are given in the text. The intensely luminescent band below the pUL17 band contains the IgY heavy chain, which reacts with the secondary conjugate.