FIG. 5.
Coimmunoprecipitation of pUL17 and VP13/14 in the presence and absence of capsids. Cells were infected with 5.0 PFU per cell of HSV-1(F), UL17-null virus, or UL18-null virus. The UL18-null virus lacks intact triplexes necessary for capsid formation. At 16 h after infection, a sample of lysates was removed for immunoblotting (upper rows), and the rest of the lysate was subjected to immunoprecipitation with the VP13/14-specific antibody (lower rows). Immunoprecipitated material was electrophoretically separated and subjected to immunoblotting with antibodies to pUL17 (3rd row) or VP13/14 (4th row).