FIG. 6.
Immunofluorescence staining of cells infected with a virus encoding an HA-tagged VP13/14. At the indicated times after infection, Hep2 cells were fixed, permeabilized, and reacted with antibodies to pUL17 (leftmost column), HA (indicating VP13/14 localization; second column from the left), or ICP4 (rightmost column). The third column from the left is a merge of the two columns to its left. Panels U, V, and W are higher magnifications of the areas within the rectangles in panels M, N, and O, respectively. The arrows in panels M, N, O, R, and S indicate regions of the plasma membrane bearing pUL17 and VP13/14-HA-specific signals. RC, replication compartment; PM, plasma membrane.