TABLE 2.
Predicted protein | No. of matching peptides | SDS-PAGE mass (Da) | Predicted MW | Accession no. | Protein scorea | Total ion scoreb | Best ion scorec | Total CId (%) |
---|---|---|---|---|---|---|---|---|
Histone H4 (mouse) | 21 | 15,000 | 12,480 | gi|27692935 | 484 | 334 | 86 | 100 |
Histone H2A (human H2Aj) | 10 | 17,500 | 13,927.80 | gi|7264004 | 240 | 187 | 70 | 100 |
Histone H3 (human) | 5 | 19,000 | 15,498.50 | gi|56204740 | 265 | 119 | 92 | 100 |
Vimentin (human) | 14 | 62,000 | 49,623.10 | gi|57471646 | 539 | 376 | 115 | 100 |
Virion protein UL47 | 39 | 85,000 | 73,771.30 | gi|9629428 | 355 | 141 | 52 | 100 |
Virion protein UL46 | 33 | 95,000 | 78,195.70 | gi|73826 | 431 | 263 | 85 | 100 |
Major capsid protein HSV1 | 52 | 160,000 | 148,989.30 | gi|9629399 | 714 | 464 | 101 | 100 |
Protein score represents the combined unique ion scores of all observed mass spectra of a protein using Mascot software.
Total ion score represents the combined ion scores of all significant peptides.
Best ion score represents the score of the highest matching peptide fragment.
CI, confidence interval.