FIG. 5.
MCMV-ΔM94 protects against challenge with MCMV-wt. B6 mice (n = 5) were immunized (1st) s.c. or i.p. with 105 TCID50 MCMV-wt (wt; solid symbols), MCMV-ΔM94 (ΔM94; open symbols), Δm01-17+m144-158-MCMV (ΔΔ; dark gray symbols), or PBS (light gray symbols). Virus preparations were UV irradiated before immunization (UV) as indicated. Optionally, mice were boosted (2nd) 2 weeks later with the same dose, route, and virus. Challenge infection was applied i.v. 20 (A) or 4 weeks (B) after priming with 106 PFU MCMV-wt. Five days postchallenge, a plaque assay was performed. Horizontal bars show the medians for the groups. Each symbol represents one individual mouse. DL, detection limit.