FIG. 7.

Antibody-secreting cells (ASC) induced by microneedle VLP vaccine. Spleen and bone marrow samples were collected from individual mice in each group (n = 6) at day 4 postchallenge. Spots representing antibody-secreting cells were determined by ELISPOT assay. Significant differences were found among the groups: in spleen (A) for MN+T versus MN, and IM+T, IM-intact, P < 0.05; for MN versus IM, P < 0.05; in bone marrow (B), for MN+T versus MN, IM+T, and IM-intact, P < 0.01; for MN versus IM, P < 0.05.