FIG. 7.

ZASC1 activates the expression of the MLV promoter. (A) Basal activity of a plasmid encoding Gaussia luciferase under the control of either the WT MLV LTR promoter or a promoter with all three ZBS mutated in transiently transfected CHO-K1 or IM1 cells. Promoter activity of the WT plasmid is set to 100% for each cell line. (B) CHO-K1 or IM1 cells transiently transfected with WT or mZBS reporter constructs with or without a ZASC1 expression plasmid. WT and mZBS promoter activity in the absence of ZASC1 in each cell line was set to 1, and fold-activation in the presence of ZASC1 was determined as described in Materials and Methods. The data shown are the average mean values obtained in an experiment performed with quadruplicate samples, and each is representative of three independent experiments. Error bars indicate the standard deviations of the data. P values were calculated using a standard Student's t test.