TABLE 1.

Oligonucleotides used for cloning shRNA expression construct

Target genea	Oligonucleotide name	Orientationb	Sequence (5′ to 3′)c	Binding positiond
SEAP	OJWB299	Sense	TGCTGCATGTCTGCTCGAAGCGGCCGGTTTTGGCCACTGACTGACCGGCCGCTGAGCAGACATG	1539
	OJWB300	Antisense	CCTGCATGTCTGCTCAGCGGCCGGTCAGTCAGTGGCCAAAACCGGCCGCTTCGAGCAGACATGC
ZASC1	OJWB309	Sense	TGCTGTTTGCTGGTTGACTGATTCAGGTTTTGGCCACTGACTGACCTGAATCACAACCAGCAAA	376
	OJWB310	Antisense	CCTGTTTGCTGGTTGTGATTCAGGTCAGTCAGTGGCCAAAACCTGAATCAGTCAACCAGCAAAC
ZASC1	OJWB546	Sense	TGCTGTTGAGGAGAACTGTACATCACGTTTTGGCCACTGACTGACGTGATGTAGTTCTCCTCAA	881
	OJWB547	Antisense	CCTGTTGAGGAGAACTACATCACGTCAGTCAGTGGCCAAAACGTGATGTACAGTTCTCCTCAAC
ZASC1	OJWB548	Sense	TGCTGATAGCAACATGCCTGTTAACAGTTTTGGCCACTGACTGACTGTTAACACATGTTGCTAT	1164
	OJWB549	Antisense	CCTGATAGCAACATGTGTTAACAGTCAGTCAGTGGCCAAAACTGTTAACAGGCATGTTGCTATC
ZASC1	OJWB550	Sense	TGCTGTTATGAGGCAAGTCAGCTGAAGTTTTGGCCACTGACTGACTTCAGCTGTTGCCTCATAA	1362
	OJWB551	Antisense	CCTGTTATGAGGCAACAGCTGAAGTCAGTCAGTGGCCAAAACTTCAGCTGACTTGCCTCATAAC

^aThe gene that the shRNA targets.

^bDenotes whether the oligonucleotide binds on the coding strand (relative to the mRNA of the genes) or noncoding strand.

^cSequence of the oligonucleotide written in the 5′ to 3′ orientation.

^dThe region of the target gene the shRNA corresponds to, relative to the start codon.