FIG. 2.

Temporal expression of the ac76 transcript in AcMNPV-infected Sf9 cells. Total RNA was extracted from mock-infected (lane Mi) and AcMNPV-infected cells at designated time points. (A) Northern blot analysis of the transcript from the ac76 region. A 59-nt single-stranded, alkaline phosphatase-labeled cRNA probe complementary to ac76 was used, and the sizes of specific hybridization bands are indicated on the right. (B) The 5′ RACE product of the ac76 transcripts. Total RNA derived from AcMNPV-infected cells at 24 h p.i. was used. The PCR product was purified and sequenced to determine the transcriptional start site. (C) Analysis by 3′ RACE of ac76 transcripts. The PCR products derived from RNA isolated at 24 h p.i. were purified and sequenced to determine the transcriptional stop site. M, DNA marker. The sizes of the PCR products are indicated on the right. (D) The transcriptional initiation and termination sites of ac76 transcripts.