FIG. 7.
Subcellular localization of the Ac76-GFP fusion protein in Sf9 cells infected with GFP-tagged virus. (A) Schematic diagram of the generation of GFP-tagged recombinant viruses. An ac76-gfp chimera, under the control of the native ac76 promoter, and polh were inserted into the polh locus of the vAcac76-KO bacmid to generate vAcac76KO-PH-Ac76GFP. The control virus vAcPH-p76GFP was constructed by transposing gfp (under the control of the ac76 promoter) and polh into the AcMNPV bacmid. (B) Confocal images of Sf9 cells infected with recombinant virus. Cells were infected with vAcac76-KO-PH-Ac76GFP at an MOI of 10 and were observed for fluorescence by confocal laser scanning microscopy at 12, 24, 48, and 72 h p.i. As a control, cells were infected with vAcPH-p76GFP and examined with the confocal microscope. For each time point, GFP-specific fluorescence micrographs are shown to the left of the merged micrographs. Scale bar, 10 μm.