FIG. 2.
Transcriptional profile and R. typhi burden during various stages of growth in Vero76 cells. The mean normalized expression (±standard errors) of RT0522 was calculated relative to the expression of the rickettsial reference (rpsL) gene. To account for rickettsial growth in host cells at different stages of the rickettsial life cycle, the mean rickettsial burden (±standard errors) was calculated by dividing the efficiency-corrected CT values for the RT0119 rickettsial housekeeping gene (rpsL) by that of the host housekeeping gene (GAPDH). The expression levels of RT0522 at all time points were found to be significantly different (P < 0.05 [two-tailed t test]) from that of the 8-h-postinfection time point.