FIG. 4.

Cytotoxicity in yeast transformants determined by CFU assay. The percentages of CFU in yeast cells transformed with LacZ (pYES/CT/LacZ/INVSc1, for control plasmid), RT0522 (pYES-522HS/INVSc1, which showed no growth on inducing agar), S86A (pYES-S86A/INVSc1), and S86A-D250A (pYES-S86A-D250A/ INVSc1) were determined as described in Materials and Methods. MAPF and BEL represent yeast cells transformed with RT0522 (pYES-522HS/INVSc1) that were plated onto inducing (SC-U+Gal) agar containing PLA₂ inhibitors MAPF at 15 μM and BEL at 20 μM, respectively.