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. 2010 Apr 30;192(13):3294–3303. doi: 10.1128/JB.00155-10

FIG. 5.

FIG. 5.

Cytotoxicity assay of RT0522 expression in Vero76 cells. (A) Western blot analysis of the expression of RT0522 in Vero76 cells. Total proteins from Vero76 cells carrying the appropriate plasmid were probed with anti-RT0522 antibody. Lane 1, MagicMark XP Western protein standard (Invitrogen); lane 2, pcDNA-522HS/Vero76; lane 3, pcDNA6.2/N-EmGFP/GW/CAT/Vero76 (for control plasmid). The size of the expected protein RT0522 (including N-terminally tagged EmGFP protein) in lane 2 is shown on the right (94.9 kDa). (B) The cytotoxicity of RT0522 expression in Vero76 cells was measured by LDH release assay and is presented as the percentage of lysed Vero76 cells. RT0522, pcDNA-522HS/Vero76; ExoU, pcDNA-ExoU/Vero76; CAT, pcDNA6.2/N-EmGFP/GW/CAT/Vero76. Error bars represent the standard errors of the means. The cytotoxicities of RT0522 and ExoU were found to be significantly different (P < 0.05 [two-tailed t test]) from that of the CAT control plasmid. The cytotoxicity of ExoU was significantly different (P < 0.05 [two-tailed t test]) from that of RT0522.