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. 2010 Apr 30;192(13):3294–3303. doi: 10.1128/JB.00155-10

FIG. 7.

FIG. 7.

Inhibition of phospholipase A (PLA) activity of RT0522 by methyl arachidonyl fluorophosphonate (MAPF). The inhibitor MAPF at an 0.1 μM or 0.5 μM final concentration (dissolved in dimethyl sulfoxide [DMSO]) was added to a reaction mixture containing recombinant protein RT0522 (from pTrc-522HS), fluorogenic phospholipid substrate, and Vero76 cell lysate as described in Materials and Methods. The PLA activity measured as fluorescence intensity/μg of recombinant proteins is presented as a percentage of RT0522 PLA activity. Error bars represent standard errors of the means. The % PLA activity of RT0522 (dimethyl sulfoxide) was found to be significantly different (P < 0.05 [two-tailed t test]) from that in the presence of 0.1 μM or 0.5 μM MAPF.