TABLE 2.

Synthetic oligonucleotides used in PCR amplification and primer extension

Oligonucleotide	Sequence (5′-3′)a	Restriction site	Annealing positionsb
gerR2s	ggatccGAAGAAAAGATTGCGCCG	BamHI	−293 to −275
ylBOaBamHI	ggatcc TGAAAGCTGTGTTCCTCC	BamHI	+99 to +82
ylBOsenso	AGCCTTTGAGGAGGTTGG		+99 to +116
ylBOanti	CATGATATCGATCAGTGCC		+513 to +495
ylBOH3	aagcttCCGGTTCATTTCAGGCG	HindIII	+604 to +588
cotBps	gaattcTATGAACGGATTAGGCCG	EcoRI	−268 to −250
cotBpa	ggatccAATACTTTTACATGCTCC	BamHI	+206 to +187
cotAps	gaattcGTCTATCTTGTCATCGCC	EcoRI	−451 to −433
cotApa	ggatccTGTAGCCCCACAGGCG	BamHI	+154 to +139
cotDps	gaattcCAGGACAGTGCACGGC	EcoRI	−594 to −578
cotDpa	ggatccGCCATCATATGCGGTCTG	BamHI	+29 to +12
cotCps	gaattcGAATTCCGGCAGACGTAC	EcoRI	−564 to −531
cotCpa	ggatccGTATTTTTTGTAATAACC	BamHI	+11 to +4
cotGps	gaattcTGGATGAACAAACACCTG	EcoRI	−187 to −169
cotGpa	ggatccTGCGACTTTTTATGACTGC	BamHI	+118 to +99
spoVIFs	gaattcATGAGATTGAAGAGCTGG	EcoRI	−570 to −552
spoVIFa	ggatccGGCAAGATTCATTACATCC	BamHI	+71 to +52
gerR PE	CTGAGTCCAAGCATCTTG		+33 to +15
gerRcoding	tctagaATGAGATAAATAAGCTGAC	XbaI	−35 to −17
gerRanti	ctcgagCGTTTCTTGAGCCGC	XhoI	+578 to +563
chip cotUs	TATGATCTGTTTCTAGCCG		−350 to −331
chip cotU1	GAAAAACAAAACGGATAGC		−107 to −126
chip cotB	ACATAGATGATAAACATACTG		−23 to −43
yjczs	GGTCGTATTATTCATTCTCC		−154 to −134
chip spoVIF1	CGGTATTGTATGAATGCAC		−26 to −45
chip gerEs	GTATCCGTAAGATTTTCCG		−490 to −471
chip gerE1	GATATCATATGTAAGAAGGG		−60 to −80
ftsHs	CAAAGCAGACTTTGTCGG		−223 to −205
ftsHa	GTACCATAGAAAATCATTCC		−39 to −59

^aThe restriction sites inserted with synthetic oligonucleotides are shown in lowercase type.

^bConsidering the first base of the first codon as +1.