TABLE 4.
Kinetic parameters of Pi uptake in WT Synechocystis and pst1 and pst2 whole-gene-cluster deletion mutantsa
| Strain | High range (10-0.75 μM) |
Low range (250-15 nM) |
||
|---|---|---|---|---|
| Ks (μM) | Vmax (fmol cell−1 min−1) | Ks (μM) | Vmax (fmol cell−1 min−1) | |
| Wild type | 3.26 ± 0.5 | 34.15 ± 3.66 | 0.24 ± 0.05 | 3.8 ± 0.75 |
| Δpst1 mutant | — | — | 0.07 ± 0.01 | 0.88 ± 0.11 |
| Δpst2 mutant | 3.7 ± 0.7 | 31.18 ± 3.96 | — | — |
a
Maximum uptake rates (Vmax) and half-saturation constants (Ks) were obtained from five independent experiments, each performed in triplicate. The standard deviations are indicated. Dashes indicate that no measurable Pi uptake occurred during the time frame of the experiment.