FIG. 5.
Minimal requirements for transposon circle integration. (A) Structure of transposon circles assembled from IRs truncated in their internal region. The total length of the IRs forming the junctions is indicated. The integration capacity of these constructs (step 2) was assayed as described in the legend to Fig. 2 and is shown in panel B. Data were obtained from at least four parallel measurements. (C) EMSA for IR-IR junctions assembled from intact and/or truncated IRs. The first 134 aa of the IS30 Tpase and the 32P-labeled IR-IR junction fragments (26-26, 19-19, 26-19 bp) were applied in the shift assay. Open and filled arrowheads indicate the shifted complexes possibly containing one and two Tpase molecules, respectively.