FIG. 5.

EMSA of the binding of LigR to the ligK promoter region. (A) DNA fragments used for the EMSA. Binding (++, +++; thicker bars) and nonbinding (−; thinner bars) of the respective fragments are indicated. “++” or “+++” represents the observation of two or three LigR-DNA complexes, respectively. The circled M in fragments KRTN12SD and KRTN129SD indicates that the T-N₁₁-A motifs were changed by mutation. (B) EMSA with purified LigR and DIG-labeled fragments containing the ligK promoter region. The presence (+) or absence (−) of purified LigR (100 nM), PCA, and GA in the reaction mixtures is indicated. Inducers were added at a concentration of 1 mM in each reaction. The positions of complex I (CP I), complex II (CP II), complex III (CP III), and free probe (F) are indicated.