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Growth requirement of the Δmmp1527::pac mutant S600 for lysine. (A) Growth in McNA. (B) Growth in McNA containing lysine (1 mM). •, wild-type strain S2; ○, Δmmp1527 mutant S600; ▴, strain S601 (complemented strain S600 with mmp1527 expressed from pMEV2-Mmp1527). The growth curves are representative curves for experiments that were independently replicated three times. All values are the averages of triplicate cultures.
