FIG. 1.
Influence of oxidizing conditions on the oligomerization of purified His6-DcuS. Detergent-solubilized and purified variants of His6-DcuS (1 μg) were subjected to SDS-PAGE in the presence (+) or absence (−) of DTT. DcuS was detected by Western blotting with antiserum against the periplasmic domain of DcuS. The anti-DcuS-positive bands of 61 and 120 kDa correspond to monomeric and dimeric DcuS. Lanes: 1 and 2, DcuSWT; 3 and 4, DcuSCys−; 5 and 6, DcuSC471S; and 7 and 8, DcuSC199S. For calibration, PageRuler Plus prestained protein ladder (Fermentas) was used.