FIG. 6.

(A, B) Effects of CLEs on commitment and DPA release during B. subtilis spore germination. Commitment (•, ▾, ▪, ⧫) and DPA release (○, ▿, □, ⋄) of heat-activated spores of B. subtilis strains PS533 (wild type) (•,○), FB111 (cwlJ) (▾, ▿), FB112 (sleB) (▪, □), and FB113 (cwlJ sleB) (⧫, ⋄) germinating in 2 mM l-alanine (A) or 10 mM AGFK (B) were measured as described in Materials and Methods. The maximum number of RFU that each germination assay reached upon germination for 3 h in panel A and for 6 h in panel B was set at 100%, and saturating concentrations of l-alanine or AGFK were used in order to achieve maximum germination. Note that different preparations of PS533 spores were used in the experiments shown here and in Fig. 3 and 4.