TABLE 2.
Effect of heat activation on timing of commitment and DPA release and lag times between commitment and DPA release during l-alanine germination of Bacillus spores
| Spores and heat activation | Time (min)a |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| C25 | G25 | Δ25 | C50 | G50 | Δ50 | C75 | G75 | Δ75 | |
| Wild-type B. subtilis | |||||||||
| Yes | 4 | 8 | 4 | 8 | 11.8 | 3.8 | 12.6 | 17.4 | 4.8 |
| No | 7 | 12 | 5 | 11 | 17.6 | 6.6 | 18 | 24.3 | 6.3 |
| B. cereus | |||||||||
| Yes | 1.1 | 2.8 | 1.7 | 1.7 | 3.6 | 1.9 | 2.5 | 4.8 | 2.3 |
| No | 2.1 | 3.7 | 1.6 | 2.7 | 5 | 2.3 | 3.2 | 6.7 | 3.5 |
a
Ct, Gt, and Δt values (times to reach 25%, 50%, and 75% commitment [C25, C50, and C75] and germination [G25, G50, and G75] and Gt − Ct times [Δ25, Δ50, and Δ75]) were determined from data in Fig. 2A and B.