TABLE 3.

Effects of germinant concentrations on the timing of commitment and DPA release and lag times between commitment and DPA release during nutrient germination of Bacillus spores

Spores, germinant (concn [mM])	Time (min)a
	C25	G25	Δ25	C50	G50	Δ50	C75	G75	Δ75
Wild-type B. subtilis, (l-Val)
10	5	10	5	8.6	14.2	5.6	13.2	19.6	6.4
2	15	23.8	8.8	26.5	38.2	11.7	45.6	56	10.4
1	33.8	42.3	8.5	57.5	69	11.5	93	116	23
Wild-type B. subtilis, AGFK
10	20	30.5	10.5	34	56	22	70	112	42
5	22.5	37.5	15	40	73	33	85	152	67
1.5	29.2	42	12.8	52.5	86	33.5	102	165	63
B. cereus, (l-Ala)
6	0.8	2.4	1.6	1.3	2.9	1.7	1.9	3.1	1.2
1	1.6	3.4	1.9	2.3	4.3	2.0	3.7	5.6	2.0
0.3	2.2	4.2	2.0	3.1	5.4	2.3	4.12	6.8	2.7

^aC_t, G_t, and Δ_t values were determined from data in Fig. 3A to C. Different preparations of wild-type B. subtilis spores (strain PS533) were used in the experiments in Fig. 3 and 4.