TABLE 4.

Effects of elevated levels of the GerA GR on the timing of commitment and DPA release and lag times between commitment and DPA release during nutrient germination of B. subtilis spores

B. subtilis strain, germinant, concn (mM)	Time (min)a
	C25	G25	Δ25	C50	G50	Δ50	C75	G75	Δ75
Wild type, l-Val
10	17.2	26.5	9.3	26.8	39.5	12.7	38	54.2	16.2
4	28	42.5	14.5	47.5	64.5	17	72.5	93.7	21.2
1	48.5	71.8	23.3	74.8	103.5	28.7	118	155	37.5
PsspD::gerA, l-Val
10	10	17.2	7.2	21.5	28.3	6.8	32	40	8
2	24	30	6	40	47.8	7.8	55.8	64.5	8.7
0.5	33	38	5	56	65	9	93	104	11

^aC_t, G_t, and Δ_t values were determined from data in Fig. 4A and B. Note that different preparations of wild-type spores were used in the experiments in Fig. 3 and 4, and this is presumably the reason for the different C_t, G_t, and Δ_t values obtained.