TABLE 5.
Effects of elevated levels of the GerB* GR on the timing of commitment and DPA release and lag times between commitment and DPA release in B. subtilis spores
| B. subtilis strain, germinant, concn (mM) | Time (min)a |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| C25 | G25 | Δ25 | C50 | G50 | Δ50 | C75 | G75 | Δ75 | |
| FB10, l-Asn | |||||||||
| 10 | 5.3 | 11.8 | 6.5 | 11.8 | 20 | 8.2 | 17.6 | 24.4 | 6.8 |
| 1 | 8.8 | 14.6 | 5.8 | 14.2 | 24.1 | 9.9 | 25 | 42 | 17 |
| 0.25 | 8.8 | 16.3 | 7.5 | 20.3 | 31 | 10.7 | 42 | 62.5 | 20.5 |
| PS3502, l-Asn | |||||||||
| 10 | 4.4 | 7 | 2.6 | 8 | 11.8 | 3.8 | 13.4 | 18.6 | 5.2 |
| 1 | 4.8 | 8.1 | 3.3 | 9.4 | 15 | 5.6 | 16.2 | 25 | 8.8 |
| 0.25 | 5.8 | 9.8 | 4 | 11.5 | 18.1 | 6.6 | 19 | 30 | 11 |
| PS3415, l-Asn | |||||||||
| 1 | 1 | 3.8 | 2.8 | 2 | 5.2 | 3.2 | 3.4 | 7 | 3.6 |
| 0.2 | 1.5 | 5.3 | 3.8 | 4 | 7.6 | 3.6 | 7 | 10.5 | 3.5 |
| 0.05 | 2.7 | 7.6 | 4.9 | 6 | 11.6 | 5.6 | 11.7 | 19.6 | 7.9 |
a
Ct, Gt, and Δt values were determined from data in Fig. 5A and B.