TABLE 1.
Primers used in this study
| Oligonucleotide | Sequence (5′→3′) |
|---|---|
| DNA | |
| frtB-R | AATAAGCTTCCTTGTCCTAACATCCCGG |
| frtB-L | AATAGTACTTGGCACATTAGCGATCG |
| nifB1RTL | GGCAGCTAGTCCACCGACAT |
| nifB1RTR | ATCCGCAACCACCTGATTTT |
| nifH1RTL | ACAGGCGTGAGATCCAAACA |
| nifH1RTR | CATCAAACGGGTGGAGTCAG |
| nifK1RTL | CTACCTTGAGGAGGAGTGAA |
| nifK1RTR | CTCGGTGTATTCTGGCTGTT |
| nifU3′RTL | CAAAGCCGCAACAAACC |
| nifU3′RTR | GCGTAATCTGGATTCAATCG |
| nifSRTL | CATTCGTGCCGCATTGTTAGCC |
| nifSRTR | ACAGTCCCGGTTTCGTTGTTCG |
| nifH302L | GAAGATCTCAGCCTAGTAGTAGAAGCAGTT |
| nifH1-R2 | ATACCCGGGTCTAATGTTTTCGTCAGTCA |
| nifSUHL1 | TGAAGATCTAAGGTAGATCCAGAGGTTGTAGAGG |
| nifSUHL2 | TGAAGATCTAGCAATGGAATAAGGGCTAATGAG |
| nifBSUHL | TGAAGATCTAGCAACCGCGTCTGATAGTGT |
| nifUH-L | AATAGATCTAGCCCAAGAACAAACATTG |
| nifUH-L2 | AATAGATCTGGGAGTCATTGAAGATAACG |
| nifUH-L3 | AATAGATCTCTGACTTTAGATGAAGCCCTG |
| nifUH-L4 | AATAGATCTGAAAATAAGGTACGTCGCATAG |
| nifUH-L5 | AATAGATCTGGCAAAAACGACCCCTC |
| vnfHBgal-L451 | GAAGATCTTGCATCAATCAAGATATGATTTAGTGATT |
| vnfHBgal-L1380 | GAAGATCTCAGAACGCGCTTAGGGATGAG |
| vnfHBgal-L196 | GAAGATCTAAGACGTTTTCATTGTTTGG |
| vnfHBgal-R1 | ATACCCGGGATCGCGGAAGCCTTTGAGTACTACTT |
| nifBRTR2 | GCAATACGTTCTTGGAGCTTTTC |
| nifURTR | GGTCTTACTTCTTCGTCTAATACTTTTTG |
| nifBPCR1 | GGTAGAATGTGTTTACAGCCAAG |
| nifHout | CCATAGCTGCAAGGGTGTTT |
| Oligop1 | GCGCGAATTCCTGTAGA |
| vnfHPE1R | GCGGAAGCCTTTGAGTAC |
| vnfHPE2R | CCGACAATCAGAATACGTTGT |
| moe2RPE3 | AGCTATGCGTAGTGCGATCGCCACT |
| Moelike2-RPE | AGGAGGCCACTATCCTGCTT |
| nifB1L | AATGTCGACAACAAGATGATTCGGGAACAAGGTGCATTC |
| nifB1R | AATACTAGTCGGTTTCGTTGTTCGCATACATAATTGTCA |
| nifH170L | TGTACATCCGACTAACGAACCCATCATGAACA |
| nifH170R | GCTACATCTGTGATGAGTGCTGAGTCCATA |
| RNA | |
| RNAoligo09 | AUAUGCGCGAAUUCCUGUAGAACGAACACUAGAAGAAA |