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. 2010 Apr 16;192(13):3534–3539. doi: 10.1128/JB.00046-10

FIG. 3.

FIG. 3.

Secretion and translocation of native and 20EspA-Map/Tir-TEM fusions. (A) Native Map and Tir and 20EspA-Map and 20EspA-Tir fused to TEM were secreted from wild-type EPEC E2348/69 (E69); enhanced secretion of native Tir, 20EspA-Map, and 20EspA-Tir from the sepD and sepL mutants was detected. Neither protein was secreted from the escN mutant. Molecular mass markers (in kilodaltons) are indicated to the left. (B) A translocation assay using wild-type EPEC revealed that 20EspA enhanced the translocation of Map, while the translocation of 20EspA-Tir remained at the same levels as that of native Tir. Neither effector was translocated from the escN mutant. The data presented are averages of triplicate values obtained from two independent experiments. The statistical significance (*) of the difference between E69 and E69ΔescN expressing the indicated TEM fusion (horizontal lines) was determined by Student's t test.