TABLE 2.
Plasmids used in this work
| Plasmid | Relevant characteristics | Reference |
|---|---|---|
| pBBR1MCS2 | Kmr cloning vector replicable in Rhizobium | Kovach et al., 1995 (16) |
| pCR-ctRNAwt 42d | pBBR1MCS2 carrying a ctRNA wild type from p42d R. etli | This work |
| pCR-ctRNAwt SymA | pBBR1MCS2 carrying a ctRNA wild type from pSymA S. meliloti 1021 | This work |
| pCR-ctRNA-CGCU | pBBR1MCS2 carrying a ctRNA with modified loop (from 5′-GCGA to 5′-CGCU) | This work |
| pCR-ctRNAmut2-5 | pBBR1MCS2 carrying a ctRNA with nucleotides 2-5 changed in the left arm from CUAC to AAUG | This work |
| pCR-ctRNAmut2-5 CGCU | pBBR1MCS2 carrying a ctRNA with both mutations: modified loop (from 5′-GCGA to 5′-CGCU) and nucleotides 2-5 changed in the left arm from CUAC to AAUG | This work |
| pCR-ctRNA-Δ(1-8) | pBBR1MCS2 carrying a ctRNA with lack eight of the first nucleotide of the left arm | This work |
| pCR-ctRNADqS | pBBR1MCS2 carrying a ctRNA with stem-loop and 3′ end from incα region of S. meliloti pSymA and 5′ end (13 nt) from incα region of R. etli p42d | This work |
| pCR-ctRNA5N | pBBR1MCS2 carrying a ctRNA with stem-loop and 3′ end from incα region of R. etli p42d and 5′ end with nonrelated nucleotides | This work |
| pCR-ctRNAart | pBBR1MCS2 carrying a ctRNA with stem-loop with nonrelated nucleotides, 3′ end and 5′ end from ctRNA of p42d | This work |