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Localization of GFP-Ras2. Image was acquired using a 63× 1.2 na water objective with high intensity 488 nm excitation and emission collected between 500 nm and 550 nm. A) Wildtype GFP-Ras2 is mainly localized to the plasma membrane B) GFP-Ras2C318S is localized to both plasma and endomembranes.
