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Time-stamped FRAP imaging sequence acquired at 1400 lines/s using bidirectional scanning and fly-mode on a Leica TCS-SP5 CLSM: (A) Pre-bleach image (B) Image acquired during the bleach frame (C) Image acquired 137 ms after the bleach frame (D) Image acquired after 19.8 seconds of recovery. The images are presented in 256 level-inverted grayscale color map, where black represents saturation and white represents zero signal.
