Figure 4.

FRAP data, model fits and predictions: (A) GFP-Ras2 FRAP data for 1μm × 1μm (•) and 0.5μm × 0.5μm () bleach ROI’s, and model fit for 1μm × 1μm (—) bleach ROI and model prediction for 0.5μm × 0.5μm () bleach ROI using the estimated parameters D = 0.0691 μm²/s, α = 27.7 s⁻¹ for 1μm × 1μm bleach ROI, (B) GFP-Ras2C318S FRAP data for 1μm × 1μm (•) and 0.5μm × 0.5μm () bleach ROI’s, and model fit for 1μm × 1μm (—) bleach ROI and model prediction for 0.5μm × 0.5μm () bleach ROI using the estimated parameters D = 0.2743 μm²/s, α = 43.09 s⁻¹ for 1μm × 1μm bleach ROI