Figure 5.

FRAP data with model simulations for lateral membrane diffusion and exchange with cytosol. In each panel, the FRAP data for 1μm × 1μm and 0.5μm × 0.5μm bleach ROI’s are plotted using black and red dots, and the corresponding model simulations are plotted using black and red solid lines respectively. (A) GFP-Ras2 data with model predictions for diffusion and slow exchange with parameters D = 0.0691 μm²/s; α = 27.7 s⁻¹; k_on = 0.01 dm s⁻¹; k_off = 0.1 s⁻¹, (B) GFP-Ras2 data with model predictions for diffusion and fast exchange with parameters D = 0.0691 μm²/s; α = 27.7 s⁻¹; k_on = 0.1 dm s⁻¹; k_off = 1 s⁻¹, (C) GFP-Ras2C318S data with model predictions for diffusion and slow exchange with parameters D = 0.2743 μm²/s; α = 43.09 s⁻¹; k_on = 0.01 dm s⁻¹; k_off = 0.1 s⁻¹, (D) GFP-Ras2C318S data with model predictions for diffusion and fast exchange with parameters D = 0.2768 μm²/s; α = 43.5 s⁻¹; k_on = 0.1 dm s⁻¹; k_off = 1 s⁻¹